Technology
We develop and apply methods involving single-molecule fluorescence—especially single-molecule FRET—fluorescence correlation spectroscopy, and molecular simulation.
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Single-molecule Forster resonance energy transfer (smFRET)smFRET imaging provides a powerful means of visualizing the conformational and compositional dynamics of individual macromolecules and macromolecular complexes. Interrogation of individual molecules is free from ensemble averaging that would mask transient conformations. We have adapted this approach to visualize dynamic processes on the surface of—and inside of—individual virions. |
Fluorescence correlation spectroscopy (FCS)FCS provides a powerful and versatile means of probing macromolecular interactions in solution. We leverage this approach to interrogate interactions between viral proteins and RNAs, and host factors and membranes. |
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Single particle membrane fusionTo provide a functional counterpart to our investigation of conformational dynamics, we use single-particle fusion assays to monitor viral fusion to model membranes. |
Molecular dynamics (MD) simulationWe use MD simulation to aid in the interpretation of our experimental data and to inform specific hypotheses that can be tested experimentally. |
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