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Publications

2026

Unger M, Munro JB. Control of viral envelope glycoprotein function revealed by single-molecule imaging. Current Opinion in Structural Biology Accepted (2026).

Díaz-Salinas MA, Benlarbi M, Chatterjee D, Nayrac M, Robidas M, Pinyakorn S, Phanupak N, Sacdalan C, Medjahed H, Prévost J, Trautmann L, Pazgier M, Finzi A, Munro JB. Conformational dynamics of the HIV-1 envelope glycoprotein from CRF01_AE is associated with susceptibility to antibody-dependent cellular cytotoxicity. Journal of Virology 100, e0116725 (2026).

2025

Ding S, Yang D, Ullah I, Niu L, Unger M, Díaz-Salinas MA, Chandravanshi M, Zhou F, Beaudoin-Bussières G, Benlarbi M, Tolbert WD, Xu R, Laroche G, Gaudette F, Morton AJ, Lang ZL, Son A, Abrams C, Côté M, Smith AB, Huang RK, Matthies D, Munro JB*, Pazgier M*, Uchil P*, Finzi A*. Optimization of VE607 to generate analogs with improved neutralization activities against SARS-CoV-2 variants. Journal of Virology 99, e0103425 (2025). *corresponding authors

Richard J, Grunst MW, Niu L, Díaz-Salinas MA, Tolbert WD, Marchitto L, Zhou F, Bourassa C, Yang D, Chiu TJ, Chen HC, Benlarbi M, Buissières GB, Gottumukkala S, Li W, Dionne K, Bélanger E, Chatterjee D, Medjahed H, Hendrickson WA, Sodroski J, Huang RK, Matthies D, Smith AB III, Mothes W*, Munro JB*, Pazgier M*, Finzi A*. The asymmetric opening of HIV-1 Env by a potent CD4 mimetic enables anti-coreceptor binding site antibodies to mediate ADCC. Nature Communications 16, 10419 (2025). *corresponding authors

Huang QJ, Kim R, Song K, Grigorieff N, Munro JB, Schiffer CA, Somasundaran M. Virion-associated influenza hemagglutinin clusters upon sialic acid binding visualized by cryo-electron tomography. Proceedings of the National Academy of Sciences of the USA 122, e2426427122 (2025).

Durham ND, Jain A, Howard A, Luban J, Munro JB. Molecular basis for the increased fusion activity of the Ebola virus glycoprotein epidemic variant A82V: insights from simulations and experiments. Cell Reports 44, 115521 (2025).

2024

Marchitto L, Richard J, Prévost J, Tauzin A, Yang D, Chiu T, Chen HC, Díaz-Salinas MA, Nayrac M, Benlarbi M, Beaudoin-Bussières G, Anand SP, Dionne K, Bélanger É, Chatterjee D, Medjahed H, Bourassa C, Tolbert WD, Hahn BH, Munro JB, Pazgier M, Smith AB 3rd, Finzi A. The combination of three CD4-induced antibodies targeting highly conserved Env regions with a small CD4-mimetic achieves potent ADCC activity. Journal of Virology 98, e0101624 (2024).

Dey S, Pahari P, Mukherjee S, Munro JB, Das DK. Conformational Dynamics of SARS-CoV-2 Omicron Spike Trimers During Fusion Activation at Single Molecule Resolution. Structure 32, 1910 (2024).

Díaz-Salinas MA, Jain A, Durham ND, Munro JB. Single-molecule imaging reveals allosteric stimulation of SARS-CoV-2 spike receptor binding domain by host sialic acid. Science Advances 10, eadk4920 (2024).

Ebola Virus Glycoprotein Strongly Binds to Membranes in the Absence of Receptor Engagement

Vaknin A, Grossman A, Durham ND, Lupovitz I, Goren S, Golani G, Roichman Y, Munro JB, Sorkin R
ACS Infectious Diseases 10, 1590

2023

hACE2-Induced Allosteric Activation in SARS-CoV versus SARS-CoV-2 Spike Assemblies Revealed by Structural Dynamics

Chen C, Zhu R, Hodge EA, Díaz-Salinas MA, Nguyen A, Munro JB, Lee KK
ACS Infectious Diseases 9, 1180

Detergent modulates the conformational equilibrium of SARS-CoV-2 Spike during cryo-EM structural determination

Egri SB, Wang X, Díaz-Salinas MA, Luban J, Dudkina NV, Munro JB & Shen K
Nature Communications 14: 2527

(Preprint) S:D614G and S:H655Y are gateway mutations that act epistatically to promote SARS-CoV-2 variant fitness

Yurkovetskiy L, Egri S, Kurhade C, Diaz-Salinas MA, Jaimes JA, Nyalile T, Xie X, Choudhary MC, Dauphin A, Li JZ, Munro JB, Shi P-Y, Shen K, Luban J
bioRxiv 2023.03.30.535005

Regulation of Ebola GP conformation and membrane binding by the chemical environment of the late endosome

Jain A, Govindan R, Berkman A, Luban J, Díaz-Salinas MA, Durham ND, Munro JB
PLoS Pathogens 19, e1011848

2022

Restriction of Influenza A Virus by SERINC5

Lai KK, Munro JB, Shi G, Majdoul S, Compton AA, Rein A
mBio 13: e0292322

Conformational dynamics and allosteric modulation of the SARS-CoV-2 spike

Díaz-Salinas MA, Li Q, Ejemel M, Yurkovetskiy L, Luban J, Shen K, Wang Y, Munro JB
Elife. Mar 24;11:e75433

2021

Antibody affinity as a driver of signal generation in a paper-based immunoassay for Ebola Virus Surveillance

Murray LP, Govindan R, Mora AC, Munro JB, & Mace CR
Analytical and Bioanalytical Chemistry 413: 3695

A highly homogeneous polymer composed of tetrahedron-like monomers for high-isotropy expansion microscopy

Gao R, Yu CC, Gao L, Piatkevich KD, Neve RL, Munro JB, Upadhyayula S, & Boyden E
Nature Nanotechnology 16: 698

Stability and conformation of the dimeric HIV-1 genomic RNA 5’UTR

Blakemore RJ, Burnett C, Swanson C, Kharytonchyk S, Telesnitsky A, and Munro JB
Biophysical Journal 120: 4874

2020

Real-Time Analysis of Individual Ebola Virus Glycoproteins Reveals Pre-Fusion, Entry-Relevant Conformational Dynamics

Durham ND, Howard AR, Govindan R, Senjobe F, Fels JM, Diehl WE, Luban J, Chandran K, & Munro JB
Viruses 12: 1

Conformational changes in the Ebola virus membrane fusion machine induced by pH, Ca2+, and receptor binding

Das DK, Bulow U, Diehl WE, Durham ND, Senjobe F, Chandran K, Luban J, & Munro JB
PLoS Biology 18: e3000626

Structural and functional analysis of the D614G SARS-CoV-2 spike protein variant

Yurkovetskiy L, Wang X, Pascal KE, Tomkins-Tinch C, Nyalile TP, Wang Y, Baum A, Diehl WE, Dauphin A, Carbone C, Veinotte K, Egri SB, Schaffner SF, Lemieux JE, Munro JB, Rafique A, Barve A, Sabeti PC, Kyratsous CA, Dudkina NV, Shen K, & Luban J
Cell 183: 739

Acidic pH triggers lipid mixing mediated by Lassa virus GP

Bulow U, Govindan R, & Munro JB
Viruses 12: 716

Gp41-targeted antibodies restore infectivity of a fusion-deficient HIV-1 envelope glycoprotein

Joshi VR, Newman RM, Pack ML, Power KA, Munro JB, Okawa K, Madani N, Sodroski JG, Schmidt AG, & Allen TM
PLoS Pathogens 16: e1008577

2019

Associating HIV-1 envelope glycoprotein structures with states on the virus observed by smFRET

Lu M, Ma X, Castillo-Menendez LR, Gorman J, Alsahafi N, Ermel U, Terry DS, Chambers M, Peng D, Zhang B, Zhou T, Reichard N, Wang K, Grover JR, Carman BP, Gardner MR, Nikić-Spiegel I, Sugawara A, Arthos J, Lemke EA, Smith AB, Farzan M, Abrams C, Munro JB, McDermott AB, Finzi A, Kwong PD, Blanchard SC, Sodroski JG, Mothes W
Nature 568: 415-419

An asymmetric opening of HIV-1 Env is required for anti-cluster A antibody binding

Alsahafi N, Bakouche N, Kazemi M, Richard J, Ding S, Bhattacharyya S, Das D, Anand SP, Prévost J, Tolbert WD, Lu H, Medjahed H, Delgado GGO, Kirk S, Melillo B, Mothes W, Sodroski J, Smith AB, Kaufmann DE, Wu X, Pazgier M, Rouiller I, Finzi A, & Munro JB
Cell Host & Microbe 25: 578-587

Developments in single-molecule and single-particle fluorescence-based approaches for studying viral envelope glycoproteins and membrane fusion

Howard AR, & Munro JB
Advances in Virus Research 104: 124-146

Intrinsic conformational dynamics of the HIV-1 genomic RNA 5’ UTR

Brigham BS, Kitzrow JP, Reyes JPC, Musier-Forsyth, & Munro JB
Proceedings of the National Academy of Sciences of the USA 116: 10372-10381

2018

(PATENT) FRET-based reagents and methods for identifying anti-HIV compounds

Blanchard SC, Mothes W, & Munro JB
US Patent 9,938,324

HIV-1 Env trimer opens through an asymmetric intermediate in which individual protomers adopt distinct conformations

Ma X, Lu M, Gorman J, Terry DS, Hong X, Zhou Z, Zhao H, Altman RB, Arthos J, Blanchard SC, Kwong PD, Munro JB, & Mothes W
eLife 7: e34271

Probing structural variation and dynamics in the HIV-1 Env fusion glycoprotein

Munro JB, & Lee KK
Current HIV Research 16: 5-12

Direct visualization of the conformational dynamics of single hemagglutinin trimers

Das DK, Govindan R, Nikic-Spiegel I, Krammer F, Lemke EA, & Munro JB
Cell 174: 926-937

HIV Env on lockdown

Munro JB
Cell Host & Microbe 23: 699-701

2016

(PATENT) Computer-implemented platform for automated fluorescence imaging and kinetic analysis

Blanchard SC, Terry DS, Munro JB, & Geggier PE
US Patent 9,501,822

An Intermediate state of the HIV-1 envelope glycoproteins on the virus entry pathway

Herschhorn A, Ma X, Gu C, Castillo-Menendez L, Melillo B, Smith AB, Blanchard SC, Munro JB, Finzi A, Mothes W, Sodroski J
mBio 7: e01598

Comparison of Three Indirect Immunoassay Formats on a Common Paper-Based Microfluidic Device Architecture

Fernandez SC, Logounov GS, Munro JB, & Mace CR
Analytical Methods 8: 5204

2015

Crystal structure, conformational fixation, and receptor interactions of mature unliganded HIV-1 Env

Kwon YD, Pancera M, Acharya P, Georgiev IS, Crooks ET, Gorman J, Joyce MG, Guttman M, Ma X, Narpala S, Soto C, Terry DS, Yang Y, Zhou T, Ahlsen G, Bailer RT, Chambers M, Chuang GY, Doria-Rose NA, Druz A, Hallen MA, Harned A, Kirys T, Louder MK, O’Dell S, Ofek G, Osawa K, Prabhakaran M, Sastry M, Stewart-Jones GBE, Stuckey J, Thomas PV, Tittley T, Williams C, Zhang B, Zhao H, Zhou Z, Donald BR, Lee LK, Zolla-Pazner S, Baxa U, Schön A, Freire E, Shapiro L, Lee KK, Arthos J, Munro JB, Blanchard SC, Mothes W, Binley JM, McDermott AB, Mascola JR, Kwong PD
Nature Structural and Molecular Biology 22: 522-531

Structure and dynamics of the native HIV-1 Env trimer

Munro JB, & Mothes W
Journal of Virology 89: 5752-5755

2014

A conformational transition observed in single HIV-1 Gag molecules during in vitro assembly of virus-like particles

Munro JB, Nath A, Farber M, Datta SAK, Rein A, Rhoades E, & Mothes W
Journal of Virology 88: 3577-3585
2014

Highly active anti-retroviral therapies are effective against the high multiplicity of infection observed during HIV-1 cell-to-cell transmission

Agosto LM, Zhong P, Munro JB, & Mothes W
PLoS Pathogens 10: e1003982

TRIM15 is a focal adhesion protein required focal adhesion disassembly

Uchil PD, Pawliczek T, Reynolds TD, Ding S, Hinz A, Munro JB, Fang H, Floyd RW, Yang H, Xiong Y, Bewersdorf J, & Mothes W
Journal of Cell Science 127: 3928-3942

The HIV-1 Env trimer in HD

Munro JB, & Mothes W
Structure 22: 935

Structure and immune recognition of a mature HIV-1 Env trimer

Pancera M, Zhou T, Druz A, Georgiev IS, Soto C, Gorman J, Acharya P, Ofek G, Stewart-Jones GBE, Stuckey J, Bailer RT, Chuang GY, Joyce MG, Yang Y, Zhang B, Louder MK, Mascola JR, Cohen MS, Morris L, Tumba N, Haynes BF, Huang J, Connors M, Munro JB, Blanchard SC, Mothes W, & Kwong PD
Nature 514: 455-461

Conformational dynamics of single HIV-1 Env trimers on the surface of native virions

Munro JB, Gorman J, Ma X, Zhou Z, Arthos J, Burton DR, Koff WC, Coulter JC, Smith AB, Kwong PD, Blanchard SC, & Mothes W
Science 346: 759-763

2013

Cell-to-cell transmission of viruses

Zhong P, Agosto LM, Munro JB, & Mothes W
Current Opinion in Virology 1, 44-50

2011

(Books & Chapters) Dynamic views of ribosome function: energy landscapes and ensembles

Whitford PC, Altman RB, Geggier PE, Terry DS, Munro JB, Onuchic JN, Spahn CMT, Sanbonmatsu KY, & Blanchard SC
In The Ribosome: Structure, Function, and Dynamics. Eds. Rodnina, M.V., Wintermeyer, W., & Green, R. Springer

Cryoelectron microscopy structures of the ribosome complex in intermediate states during tRNA translocation

Fu J, Munro JB, Blanchard SC, & Frank J
Proceedings of the National Academy of Sciences of the USA 108: 4817-4821

Structure and dynamics of the mammalian ribosomal pre-translocation complex

Budkevich T, Giesebrech J, Altman RB, Munro JB, Mielke T, Nierhaus KH, Blanchard SC, & Spahn CMT
Molecular Cell 44: 214-224

2009

(Books & Chapters) Single-molecule studies of biomolecules

Dave R, Altman RB, Terry DS, Munro JB, Geggier PE, Feldman MB, & Blanchard SC
In Wiley Encyclopedia of Chemical Biology, Vol. 4. Ed. Begley, T. P. Wiley

Mitigating unwanted photophysical processes for improved single-molecule fluorescence imaging

Dave R, Terry DS, Munro JB, & Blanchard SC
Biophysical Journal 96: 2371-2381

Navigating the ribosome’s metastable free energy landscape

Munro JB, Sanbonmatsu KY, Spahn CMT, & Blanchard SC
Trends in Biochemical Sciences 34: 390-400

2008

A new view of protein synthesis: Mapping the free energy landscape of the ribosome using single-molecule FRET

Munro JB, Vaiana A, Sanbonmatsu KY, & Blanchard SC
Biopolymers 89: 565-577

2007

Identification of two distinct hybrid state intermediates on the ribosome

Munro JB, Altman RB, O’Connor N, & Blanchard SC
Molecular Cell 25: 505-517

2004

Mutations in a conserved motif inhibit single-stranded DNA binding and recombination mediator activities of bacteriophage T4 UvsY protein

Bleuit JS, Ma Y, Munro JB, & Morrical SW
Journal of Biological Chemistry 279: 6077-6086