While it is true that we do the Fragment Analyzer QC on your samples again before running them on the sequencer, we don’t recommend you skip running them yourself on the Fragment Analyzer prior to submitting them for Deep Sequencing.

When customers skip the Fragment Analyzer step on their own it is risky, because once we have the Deep Sequencing ticket it puts samples on a different path and workflow from the normal Fragment Analyzer queue. A) That may mean the QC results take longer because samples with a Fragment Analyzer ticket typically take precedence over the deep sequencing QC. B) If you decide you do not want the samples run, we might not get the message in time. Samples in the Deep Sequencing queue are moved forward in the workflow automatically. If they are run before you tell us to remove them from the queue, you will still have to pay for the Deep Sequencing run. C) When the usual workflow is changed, for example, removing your samples from the Deep Sequencing queue because you didn’t like the Fragment Analyzer results, it causes a disruption and extra work on our end.  That extra work for us usually means an extra fee for you. In addition to the extra work fee (aka withdrawal fee) you will be charged for the Fragment Analyzer step of the Deep Sequencing process.

If you feel very confident in your samples after Qubit, turn in the paper work for both Fragment Analyzer and Deep Sequencing with a 15-20 µl volume of each sample. That way your sample QC will be fast-tracked to the quicker Fragment Analyzer queue. Then after you receive the results you can let us know if you want your samples moved to the Deep Sequencing queue, and they will be logged in. This will save you the day that your Deep-Sequencing-only submission would have waited in the pick-up fridge. It will not expedite your samples in the Deep Sequencing queue once they are signed in.