GS-Preprocess is a simple, 5-argument pipeline that generates input data for the GUIDEseq Bioconductor package from raw Illumina sequencer output. For off-target profiling, Bioconductor GUIDEseq only requires a 2-line guideRNA fasta, demultiplexed BAM files of "plus"- and "minus"-strands, and Unique Molecular Index (UMI) references for each read. The latter two are produced by GS-Preprocess.
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