Kaufman Lab

The Kaufman Lab has a long-term interest in proteins that deliver histones to DNA with an emphasis on the DNA-replication linked histone deposition factor termed Chromatin Assembly Factor-1 (CAF-1). In their most recent studies, the Kaufman Lab has discovered multiple nucleolar proteins associated with the CAF-1 p150 subunit. Notably, p150 depletion causes redistribution of multiple nucleolar proteins and reduces nucleolar association with repetitive element-containing loci.  Furthermore, the nucleolar functions of p150 are separable from its interactions with the other subunits of the CAF-1 complex.  Together, these data define novel functions for a separable domain of the p150 protein, regulating protein and DNA interactions at the nucleolus. 

  • Smith et al. (2014) A separable domain of the p150 subunit of human chromatin assembly factor-1 promotes protein and chromosome associations with nucleoli. Mol Biol Cell, 25(18):2866-81
  • Matheson TD, Kaufman PD. (2015) Grabbing the genome by the NADs.  Chromosoma