The Wolfe Lab focuses on developing new tools for precise targeted gene editing. Platforms include zinc finger nucleases (ZFNs), transcription activator-like nucleases (TALENs) and the CRISPR/Cas9 system, as well as engineered chimeras incorporating multiple DNA:protein interfaces.  Together, these tools are subsequently applied in zebrafish to study gene function or in human cells to develop new therapeutic modalities.

• Gupta et al.  (2013) Targeted chromosomal deletions and inversions in zebrafish.  Genome Res, 23(6):1008-17. 
• Kok et al.  (2015) Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish. Dev Cell, 12;32(1):97-108.