The Lawson Lab uses multiple types of nuclease-based technologies to investigate gene function during vascular development.  Together with the Wolfe Lab, they were the first group to generate knockout zebrafish through the use of zinc finger nucleases (ZFN).  The Lawson Lab has subsequently used ZFNs to reveal new roles for genes during vascular development.  More recent work includes use of both TALENs and CRISPR/cas9 for the purpose of large-scale reverse genetic screening efforts in the zebrafish. 

• Meng et al. (2008) Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol, 26(6):695-701. 
• Siekmann et al. (2009) Chemokine signaling guides regional patterning of the first embryonic artery.  Genes Dev, 23(19):2272-7. 
• Kok et al.  (2015) Reverse genetic screening reveals poor correlation between morpholino-induced and mutant phenotypes in zebrafish. Dev Cell, 12;32(1):97-108.